Hepatitis B Test

Hepatitis B Testing

Hepatitis B is a liver infection caused by the HBV (hepatitis B virus). Hepatitis B testing can detect an active or past infection and determine immunity to the virus. This is achieved through a simple blood test, which plays a crucial role in diagnosing the disease, monitoring its progression, and assessing the effectiveness of vaccines.

It is a viral infection that leads to liver inflammation. It is commonly transmitted through contact with infected body fluids, particularly blood. Methods of transmission include childbirth, shared needles, and unprotected sex.

Hepatitis B can be short-term (acute)or long-term (chronic). Acute infections often resolve within six months without treatment, but approximately 5–10% of cases progress to chronic hepatitis B, which can lead to serious complications like liver failure, cancer, or damage.

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Why Hepatitis B Testing is Important

Testing is crucial for several reasons:

  • Diagnosing and Monitoring Infection: Testing determines whether an infection is acute or chronic and assesses the risk of transmission to others.
  • Tracking Progression and Treatment: Once diagnosed, regular testing monitors the condition, detects complications, and evaluates the effectiveness of treatment.
  • Evaluating Immunity: Tests can confirm if immunity has been acquired either through vaccination or recovery from a previous infection. This is particularly important for people at risk of HBV reactivation.
  • Screening of the Virus: Hepatitis B often causes no symptoms, so screening helps detect it early, reducing the risk of unknowingly spreading the infection and enabling timely treatment.
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What does hepatitis B evaluate?

Hepatitis B tests identify antigens (virus proteins) and antibodies (produced by the immune system in response to the virus). Key components include:

  • IgM Hepatitis B Core Antibody (IgM anti-HBc) This specific test detects IgM antibodies to HBV core proteins and is used to identify acute hepatitis B infections within six months of exposure.
  • Total Hepatitis B Core Antibody (anti-HBc) Anti-HBc appears in the blood as symptoms of hepatitis B develop and remain detectable for life. This test, which identifies both IgM and IgG anti-HBc antibodies, is often interpreted alongside other tests to distinguish between acute and chronic infections or to confirm recovery from a previous infection.
  • Hepatitis B Surface Antibody (anti-HBs) The immune system produces anti-HBs in response to the presence of HBsAg. Detecting these antibodies suggests recovery from hepatitis B and immunity to the virus. Anti-HBs antibodies are also found in individuals previously exposed to HBV, including those who have been vaccinated.
  • Hepatitis B Surface Antigen (HBsAg) HBsAg refers to proteins found on the surface of the hepatitis B virus (HBV). These proteins are present in high levels during both acute and chronic infections. Testing for HBsAg is commonly used to screen for, detect, and diagnose HBV infections.

Additional Tests for Monitoring and Treatment Planning

If hepatitis B is confirmed through initial testing, further evaluations may be conducted to monitor disease progression, guide treatment strategies, and assess the potential to transmit the virus:

  • Hepatitis B Viral DNA This test identifies HBV's genetic material and measures the viral load in the blood. A positive result confirms active viral replication, indicating that the individual is contagious. Monitoring HBV DNA levels is essential for evaluating the effectiveness of antiviral treatments in individuals with HBV infections that are long-term.
  • Hepatitis B e-Antigen (HBeAg) HBeAg is a protein produced by HBV, detectable in some patients with a positive HBsAg result. Testing for HBeAg helps determine the infectivity of the virus and the likelihood of spreading it to others.
  • Hepatitis B e-Antibody (anti-HBe) Anti-HBe is produced by the immune system in response to HBeAg. The transition from detectable HBeAg to anti-HBe, known as seroconversion, indicates an improvement in the condition and suggests the likelihood of long-term viral clearance. Chronic liver disease is more commonly associated with the presence of HBeAg and is less frequent when anti-HBe is detected. This test is often used to monitor short-term infections.
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Who should be tested?

Hepatitis B screening is recommended for individuals at higher risk of infection, including:

  • Individuals with sexual exposure to HBV
  • Those who are HIV-positive
  • People who have not been vaccinated against hepatitis B
  • Intravenous drug users
  • Individuals from regions with high HBV prevalence (e.g., parts of Africa, Asia, and Eastern Europe)
  • Pregnant women

Testing may also be necessary if you experience symptoms such as:

  • Yellowing of the skin or eyes (jaundice)
  • Joint or abdominal pain
  • Loss of appetite, nausea, or vomiting
  • Dark urine or pale stools
  • Fatigue or fever

Additionally, testing may follow abnormal liver function tests or to confirm immunity before or after vaccination.

Testing procedure

Hepatitis B testing is typically performed using a blood sample collected by our phlebotomist or an at-home test kit.

Before the test

You do not need any special preparation for this procedure. However, you should inform your doctor about any supplements or medications you are taking.

During the test

Blood is drawn from the arm's vein for laboratory tests. This involves locating a suitable vein, tying a tourniquet around the upper arm, cleaning the puncture site, and drawing blood using a needle.

Alternatively, a small blood sample might be collected using a finger prick, particularly in young children or for at-home kits. A bandage or gauze is applied to the puncture site, and you can resume your normal activities immediately.

Receiving results

Lab results are typically available within a few days. Results are sent online or through a mobile app for at-home kits, often accompanied by an option for professional consultation if necessary.

Understanding the test result

Hepatitis B test results are interpreted in combination to provide a comprehensive view of your infection or immunity status.

anti-HBs IgM- anti-HBc HBs Ag anti- HBc Meaning

Negative

Negative

Negative

Negative

No past or current infection; no immunity.

Positive

Not performed

Negative

Negative

Immunity due to vaccination

Negative

Positive

Positive

Positive

No immunity due to the last infection

Negative

Negative

Positive

Positive

Chronic infection

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If you are experiencing symptoms or believe you may have been exposed to hepatitis B, contact our Health Screening Clinic to book your Hepatitis B test. Early detection and management are key to protecting your health and that of others.

Frequently Asked Questions

While no test is perfect, hepatitis A testing is widely considered the most reliable method for detecting an active hepatitis A infection or confirming immunity. A positive result for IgM antibodies generally indicates a current infection. However, in individuals without symptoms, the presence of IgM antibodies may sometimes suggest a previous infection or a case without symptoms.

Hepatitis A testing requires a blood sample to detect antibodies against the virus. Blood is usually drawn from a vein in the arm or the back of the hand.

When prescribed by a doctor, the sample is typically collected in a clinic or medical facility and sent to a laboratory for analysis.

When receiving your hepatitis A test results, consider discussing the following with your doctor:

  • Does my test result indicate immunity to hepatitis A?
  • What does my test result mean?
  • Do I currently have a hepatitis A infection?
  • DoIneedanyadditionaltestsbasedonmyresults?
  • Should I consider getting a hepatitis A vaccination?

Testing hepatitis A at home is not currently available in most regions. The test requires phlebotomy(blood draw), which a medical practitioner must do in a clinical or laboratory setting.

It depends on your test result. If symptoms are drastic, further tests may be necessary to monitor liver function and assess treatment options. If you test negative for hepatitis A and lack immunity, your doctor may recommend vaccination to prevent future infection.

Costs for hepatitis A testing may vary depending on your health insurance plan. In most cases, the test is covered by insurance if ordered by a doctor, though co-pays or deductibles may apply. If you do not have insurance or if your plan doesn't cover the test, you can discuss the cost with your healthcare provider, including office visit fees, blood draw charges, and laboratory testing fees.

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